Both methods are based on immunodetection.Elisa and western blot are two types of techniques used to diagnose HIV.What are the Similarities Between Elisa and Western Blot? The antibody binds with its specific antigen and reveals the presence of the desired antigen, which is a protein. A high-quality antibody is required for the detection of the desired protein by hybridization. The membrane with the transferred protein can be used to identify a particular protein. The membrane used for western blotting is mostly made up of nitrocellulose or polyvinylidene difluoride (PVDF). Western blot is the membrane used during the blotting procedure to get the mirror image of the protein patterns in the SDS- polyacrylamide gel. Western blotting is a technique that enables the detection of a specific protein from a protein mixture. It is also a time-consuming process with a requirement of advanced equipment. Therefore, ELISA is a very specific and sophisticated technique that requires the attention of well-skilled technicians. By measuring the amount of the product, the number of antigens present in the sample can be quantified. Then these conjugated enzymes incubate with the respective substrate.Įnzyme-substrate reaction produces a measurable coloured product. Once immobilized, the antigens form complexes with antibodies linked to enzymes. In this technique, the antigens in the sample bind with the immobilized antibodies on a solid surface. It is used to detect HIV antibodies, and this test shows high sensitivity. Side by Side Comparison – Elisa vs Western Blot in Tabular FormĮLISA, which stands for enzyme-linked immunosorbent assay, was the first test designed primarily for HIV detection. Similarities Between Elisa and Western Blotĥ. Western blot detects a specific protein from a protein mixture by the use of a labelled antibody. It is an enzyme immunoassay used to detect HIV antibodies. ELISA stands for enzyme-linked immunosorbent assay, which is a plate-based assay technique. Out of different HIV tests, ELISA and Western Blot are very popular today. HIV tests detect the presence of human immunodeficiency virus ((HIV) that causes this syndrome. The key difference between Elisa and western blot is that Elisa or enzyme-linked immunoassay is a diagnostic tool that detects whether the patient has been exposed to a particular type of virus or another infectious agent while western blot is a technique which detects a specific protein from a protein sample.ĪIDS has become a global problem, and the incidence of this deadly disease has increased alarmingly in the last few decades.
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